Regioselective Hydroxylation of Stilbenes by White‐Rot Fungal P450s Enables Preparative‐Scale Synthesis of Stilbenoids

Abstract Scaling up biocatalytic reactions involving cytochrome P450 monooxygenases (P450s) is challenging due to their instability, low substrate loading, co‐factor, and oxygen requirements as well the dependency on a reductase partner, which limits integration into synthetic chemistry. Recently, study investigated frequently used bacterial P450s produce bioactive stilbenoids, extending repertoire of sustainable strategies with remarkable success. This article explores complementary application less common eukaryotic viable alternative for generating same compounds when employed Pichia pastoris ‐based whole‐cell biocatalyst. In direct comparison equivalents, recently discovered CYP5035S7 CYP5035S9 from white‐rot fungus Polyporus arcularius are shown be competitively efficient at synthesising stilbenoids preparative‐scale.